Publications

Detailed Information

Application of high throughput cell array technology to FISH: investigation of the role of deletion of p16 gene in leukemias

Cited 9 time in Web of Science Cited 12 time in Scopus
Authors

Lee, Dong Soon; Lee, Jee Hyung; Min, Hyun Chung; Kim, Tae Young; Oh, Bo Ra; Kim, Ho Young; Lee, Jong Yun; Lee, Chung Kee; Chun, Hong Gu; Kim, Hee Chan

Issue Date
2007-09-05
Publisher
Elsevier
Citation
J Biotechnol. 2007 Jan 10;127(3):355-60. Epub 2006 Jul 29.
Keywords
Acute DiseaseChronic DiseaseCytogenetic Analysis/methodsFemaleHumansLeukemia/diagnosis/*geneticsMaleMultiple Myeloma/diagnosis/*geneticsPredictive Value of TestsGene DeletionGenes, p16In Situ Hybridization, FluorescenceMicroarray Analysis
Abstract
Bio-cell chip is a chip that has hundreds of types of cells arrayed and immobilized on a small slide. To elucidate the role of deletion of the p16 gene in hematologic malignancies, the bio-cell chip technique was applied to fluorescent in situ hybridization (FISH) study. We made a bio-cell chip with bone marrow specimen from 109 patients with acute lymphoblastic leukemia (ALL), 102 patients with acute myelogenous leukemia (AML), 47 patients with chronic myelogenous leukemia (CML), and 25 patients with multiple myeloma (MM). A glass slide with 96 separated areas was fabricated, onto which was added methanol/acetic acid fixed cell suspensions for high-throughput FISH for p16. With the successful application of bio-cell chip technique, we found that the deletion of p16 contributed to the oncogenesis in acute leukemia, but not in chronic leukemia. In conclusion, the bio-cell chip, a cell version of ultrahigh-throughput technology, was successfully applied to the FISH study, which can be utilized efficiently in the molecular cytogenetic investigation of hematologic malignancies.
ISSN
0168-1656 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16949174

https://hdl.handle.net/10371/15546
DOI
https://doi.org/10.1016/j.jbiotec.2006.07.019
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share