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The expression of TIMPs in cryo-preserved and freeze-dried amniotic membrane
DC Field | Value | Language |
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dc.contributor.author | Koh, Jae Woong | - |
dc.contributor.author | Shin, Young Joo | - |
dc.contributor.author | Oh, Joo Youn | - |
dc.contributor.author | Kim, Mee Kum | - |
dc.contributor.author | Ko, Jung Hwa | - |
dc.contributor.author | Hwang, Jeong Min | - |
dc.contributor.author | Wee, Won Ryang | - |
dc.contributor.author | Lee, Jin Hak | - |
dc.date.accessioned | 2009-11-26T02:40:56Z | - |
dc.date.available | 2009-11-26T02:40:56Z | - |
dc.date.issued | 2007-09-14 | - |
dc.identifier.citation | Curr Eye Res. 2007 Jul-Aug;32(7-8):611-6. | en |
dc.identifier.issn | 0271-3683 (Print) | - |
dc.identifier.uri | http://www.informaworld.com/smpp/content~db=all?content=10.1080/02713680701459441 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17852184 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15677 | - |
dc.description.abstract | PURPOSE: To investigate the change of tissue inhibitor of metalloproteinase (TIMP) in cryopreserved amniotic membranes (AM) according to preservation time, and to evaluate the expression of TIMP in freeze-dried AM. METHODS: Cryopreserved or fresh AMs were incubated in dispase II for two hours at 37 degrees C and their epithelial cells were scraped with a cell scraper. Remaining stromal AM was minced and frozen in liquid nitrogen, and then treated with 0.1% diethyl pyrocarbonate. The mRNA levels of TIMP-1 and -2 were determined by reverse transcription-polymerase chain reaction (RT-PCR) in epithelial and stromal cells of fresh AM, AMs cryopreserved for 6 and 12 months, and freeze dried AM, respectively. Western blot analysis and immunohistochemical staining were performed to assess the expression of TIMP-1 in fresh, cryopreserved, and freeze dried AMs. RESULTS: RT-PCR revealed that mRNAs of TIMP-1 and -2 were expressed in the amniotic epithelial cells of both fresh and cryopreserved AMs, while the stromal cells of fresh or cryopreseved AMs and freeze-dried AM showed higher expression of TIMP-1 than TIMP-2 mRNA. On Western blot analysis, the level of TIMP-1 was more in fresh AMs than in cryopreserved or freeze-dried AM, but it was not statistically significant. CONCLUSION: TIMP-1 was expressed in cryopreserved AMs until 12 months, and the amount of expression was comparable to that in fresh AMs. | en |
dc.language.iso | en | - |
dc.publisher | Taylor & Francis | en |
dc.subject | Amnion/*metabolism | en |
dc.subject | Blotting, Western | en |
dc.subject | Epithelial Cells/metabolism | en |
dc.subject | Freeze Drying | en |
dc.subject | Gene Expression/physiology | en |
dc.subject | Humans | en |
dc.subject | Immunoenzyme Techniques | en |
dc.subject | RNA, Messenger/metabolism | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Stromal Cells/metabolism | en |
dc.subject | Tissue Inhibitor of Metalloproteinase-1/*genetics/metabolism | en |
dc.subject | Tissue Inhibitor of Metalloproteinase-2/*genetics/metabolism | en |
dc.subject | Cryopreservation | - |
dc.subject | Tissue Preservation | - |
dc.title | The expression of TIMPs in cryo-preserved and freeze-dried amniotic membrane | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 고재웅 | - |
dc.contributor.AlternativeAuthor | 신영주 | - |
dc.contributor.AlternativeAuthor | 오주연 | - |
dc.contributor.AlternativeAuthor | 김미금 | - |
dc.contributor.AlternativeAuthor | 고정화 | - |
dc.contributor.AlternativeAuthor | 황정민 | - |
dc.contributor.AlternativeAuthor | 위원량 | - |
dc.contributor.AlternativeAuthor | 이진학 | - |
dc.identifier.doi | 10.1080/02713680701459441 | - |
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