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Adhesion complex in cultivated limbal epithelium on amniotic membrane after in vivo transplantation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mee Kum | - |
dc.contributor.author | Heo, Jang Won | - |
dc.contributor.author | Lee, Jae Lim | - |
dc.contributor.author | Wee, Won Ryang | - |
dc.contributor.author | Lee, Jin Hak | - |
dc.date.accessioned | 2009-11-26T02:49:03Z | - |
dc.date.available | 2009-11-26T02:49:03Z | - |
dc.date.issued | 2005-08-20 | - |
dc.identifier.citation | Curr Eye Res. 2005 Aug;30(8):639-46. | en |
dc.identifier.issn | 0271-3683 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16109643 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15689 | - |
dc.description.abstract | PURPOSE: To investigate adhesion complex formation in cultivated human limbal epithelium after transplantation into the limbal deficient model. METHODS: Cultivated epithelium on amniotic membrane was transplanted into limbal deficient rabbits. The transplanted rabbits and the controls were sacrificed at 1, 2, 3, and 4 weeks. The adhesion complex was examined by electron microscopy and immunohistochemistry. RESULTS: Morphologically identifiable hemidesmosomes appeared at 1 week, and matured adhesion complex was found at 3 weeks. Collagen VII was partly stained after transplantation. The mean numbers of hemidesmosomes/2.25 microm were 2.3 +/- 0.9, 2.5 +/- 0.5, 5.2 +/- 1.0, and 4.0 +/- 0.9 at 1, 2, 3, and 4 weeks, and all they were smaller than those in the control, respectively (p < 0.05). It reached 137.4% of the density of hemidesmosomes in human cornea at 3 weeks. The average depths of anchoring fibril were 0.10 +/- 0.03, 0.27 +/- 0.06, 0.45 +/- 0.06, and 0.46 +/- 0.12 microm at 1, 2, 3, and 4 weeks, reaching 75.0% of that in the human cornea after 3 weeks, although they were shallower than that of the control, respectively (p < 0.05). CONCLUSIONS: Assembly of adhesion complex in cultivated epithelium transplanted in limbal deficient rabbit might recover to the level of that in the human after 3 weeks, although it was delayed compared with that in normal wound healing of the rabbit. | en |
dc.language.iso | en | - |
dc.publisher | Taylor & Francis | en |
dc.subject | Animals | en |
dc.subject | Cell Adhesion/*physiology | en |
dc.subject | Cell Transplantation | en |
dc.subject | Cells, Cultured | en |
dc.subject | Collagen Type VII/metabolism | en |
dc.subject | Corneal Diseases/*metabolism/pathology/surgery | en |
dc.subject | Epithelial Cells/cytology/*physiology/transplantation | en |
dc.subject | Epithelium, Corneal/cytology/*physiology/transplantation | en |
dc.subject | Hemidesmosomes/metabolism | en |
dc.subject | Humans | en |
dc.subject | Immunoenzyme Techniques | en |
dc.subject | Limbus Corneae/*cytology | en |
dc.subject | Rabbits | en |
dc.subject | Amnion | - |
dc.subject | Stem Cell Transplantation | - |
dc.title | Adhesion complex in cultivated limbal epithelium on amniotic membrane after in vivo transplantation | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김미금 | - |
dc.contributor.AlternativeAuthor | 허장원 | - |
dc.contributor.AlternativeAuthor | 이재림 | - |
dc.contributor.AlternativeAuthor | 위원량 | - |
dc.contributor.AlternativeAuthor | 이진학 | - |
dc.identifier.doi | 10.1080/02713680590968277 | - |
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