S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Neurology (신경과학교실) Journal Papers (저널논문_신경과학교실)
Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model
- Lee, Soon-Tae; Chu, Kon; Park, Jung-Eun; Lee, Kyungmi; Kang, Lami; Kim, Seung U; Kim, Manho
- Issue Date
- Neurosci Res. 2005 Jul;52(3):243-9.
- Animals; Apomorphine/pharmacology; Behavior, Animal/drug effects/physiology; Cell Count/methods; Cells, Cultured; Corpus Striatum/drug effects/metabolism/pathology; DNA-Binding Proteins/metabolism; Disease Models, Animal; Dopamine and cAMP-Regulated Phosphoprotein 32; Drosophila Proteins/metabolism; Endogenous Retroviruses/genetics; Functional Laterality; Galactosides/diagnostic use; Glial Fibrillary Acidic Protein/metabolism; Humans; Huntington Disease/chemically induced/*therapy; Immunohistochemistry/methods; Indoles/diagnostic use; Infusions, Intravenous/*methods; Male; Motor Activity/drug effects/physiology; Nerve Tissue Proteins/metabolism; Neurons/*physiology; Parvalbumins/metabolism; Phosphoproteins/metabolism; Phosphopyruvate Hydratase/metabolism; Quinolinic Acid; RNA, Messenger/biosynthesis; Random Allocation; Rats; Rats, Sprague-Dawley; Recovery of Function/*physiology; Reverse Transcriptase Polymerase Chain Reaction/methods; Rotarod Performance Test/methods; Stem Cell Transplantation/*methods; Stem Cells/*physiology; Time Factors; gamma-Aminobutyric Acid/metabolism
- An animal model induced by striatal quinolinic acid (QA) injection shows ongoing striatal degeneration mimicking Huntington's disease. To study the migratory ability and the neuroprotective effect of human neural stem cells (NSCs) in this model, we transplanted NSCs (5 x 10(6)) or saline intravenously at 7 days after unilateral QA injection. NSCs-group exhibited the reduced apomorphine-induced rotation and the reduced striatal atrophy compared to the control. PCR analysis for the human-specific ERV-3 gene supported an evidence of the engraftment of human NSCs in the rat brain. X-gal+ cells were found in and around the damaged striatum and migrated NSCs differentiated into neurons and glias. This result indicates that intravenously injected human NSCs can migrate into the striatal lesion, decrease the following striatal atrophy, and induce long-term functional improvement in a glutamate toxicity-induced striatal degeneration model.
- 0168-0102 (Print)
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