S-Space College of Medicine/School of Medicine (의과대학/대학원) Neurosurgery (신경외과학전공) Journal Papers (저널논문_신경외과학전공)
Induction method of tyrosine kinase A-mediated cell death in rat pheochromocytoma
- Ahn, Jin-Young; Kang, Lami; Kim, Hyun-Jeong; Kang, Hyejin; Kim, Sung-Hoon; Kim, Je-Yong; Kim, Seung Ki; Wang, Kyu-Chang; Cho, Byung Kyu; Lee, Kwang-Woo; Roh, Jae-Kyu; Kim, Manho
- Issue Date
- Springer Verlag
- Biotechnol Lett. 2005 Apr;27(8):583-7.
- Animals; Apoptosis/*drug effects; Blotting, Western; Carbazoles/pharmacology; Cell Survival/drug effects; Culture Media, Serum-Free/pharmacology; Enzyme Inhibitors/pharmacology; Indole Alkaloids; Nerve Growth Factor/*pharmacology; PC12 Cells; Pheochromocytoma/metabolism/pathology; Rats; Receptor, trkA/antagonists & inhibitors/*metabolism
- Nerve Growth Factor (NGF) is a neurotrophic factor that prevents apoptosis in neuronal progenitor cells. In rat pheochromocytoma (PC12) cells, tyrosine kinase A receptor (TrkA) mediates neurotrophic or protective effects, while p75 neurotrophin receptor (p75NTR) functions as a death receptor. We have determined whether TrkA mediates any cytotoxic effect. Following serum deprivation, TrkA expression increased 2.2-fold and apoptosis began with expression of Bax proapoptotic protein. Application of NGF halved cell viability but this was reversed by K252a, the TrkA inhibitor. These results confirmed the paradoxical cytotoxic effect of neurotrophic NGF via TrkA in PC12 cells following serum deprivation.
- 0141-5492 (Print)
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