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Analytical and Electroanalytical Applications of Filamentous Bacteriophages and Development of Sensitivity-Tunable Ion Sensing Platform based on Reverse Electrodialysis : 섬사상 박테리오파지의 분석∙전기분석적 응용 및 역전기투석을 이용한 민감도 조절 가능 이온 측정 플랫폼의 개발
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 정택동 | - |
dc.contributor.author | 노지헌 | - |
dc.date.accessioned | 2019-10-21T03:44:19Z | - |
dc.date.available | 2021-09-23T07:51:48Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.other | 000000156613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/162460 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr/common/orgView/000000156613 | ko_KR |
dc.description | 학위논문(박사)--서울대학교 대학원 :자연과학대학 화학부,2019. 8. 정택동. | - |
dc.description.abstract | Chapter 1: Analytical and Electroanalytical Applications of Filamentous Bacteriophages
Filamentous bacteriophage is one of the most highlighted biomaterial, thanks to its unique properties: 1) Easy modification of its major coat protein with genetic engineering and bioconjugation, 2) The regularity of the virions along with large population and fast generation time, and 3) The high aspect ratio, 7 nm in width and 880 nm in length, which make it an excellent biomaterial for nanomaterial applications. First, filamentous bacteriophages are applied as a scaffold of antibody immobilization for bead-based 3D suspension multiplex immunoassay. The size and the fluorescence signal of virus-tethered gold microbead is measured at a DC impedance-based flow cytometer. The large quantity of antibody loading and flexible movement of filamentous virus enabled signal enhancement, elevating sensitivity by up to 5.7-fold compared to the beads without virus. This system successfully accomplished multiplex immunoassay involving four biomarkers: prostate specific antigen, cardiac troponin I, myoglobin, and creatine kinase MB in undiluted human serum. Second, filamentous bacteriophages are genetically engineered to display 3,4-dihydroxyl-L-phenylalanine (DOPA) onto the major coat protein, where cobalt oxide-based oxygen evolution catalyst (Co-OEC) are deposited spontaneously. It was demonstrated that the catalyst formed on the virus shows improved durability and decrease oxidation state compared with electrodeposited Co-OEC. The DOPA-displaying virus proposes unconventional way of generating electrocatalysts by introducing DOPA as a nucleation site as well as a dopant for Co-OEC. Chapter 2: Development of Sensitivity-Tunable Ion Sensing Platform based on Reverse Electrodialysis Reverse elecrodialysis (RED) is a salinity gradient-based power generation system, of which the output voltage is proportional to the 1) concentration difference between river water and seawater, and 2) the number of stacks comprising RED. Inspired by those features mentioned above, RED-based ion sensing platform is proposed. The cation exchange membranes and anion exchange membranes constituting traditional RED are substituted to home-made ion- selective membranes and reference membranes, which generate sample dependent and independent potential, respectively, and the sensitivity is freely modulated by changing the number of stacks. This sensing platform is integrated with a colorimetric detection system based on polyaniline, which is a well-known electrochromic dye, electrodeposited on one bipolar electrode to display sample concentration-dependent colors. Combined with the colorimetric detection system, the RED-based ion sensing platform serves as a power generator as well as an ion concentration selective signal producer, which realize self-powered and disposable ion sensor with tunable sensitivity | - |
dc.description.tableofcontents | Abstract i
Table of contents iii List of Tables v List of Figures v Chapter 1. Analytical and Electroanalytical Applications of Filamentous Bacteriophages 1 1.1 Introduction 1 1.2. Multiplex immunoassay using virus-tethered gold micro-bead by DC impedance-based cytometry 2 1.2.1. Introduction 2 1.2.2. Experimental 5 1.2.3. Result and Discussion 11 1.2.4. Conclusion 23 1.3. Chemically Deposited Cobalt-Based Oxygen-Evolution Electrocatalysts on DOPA-Displaying Filamentous Bacteriophages 25 1.3.1. Introduction 25 1.3.2. Experimental 26 1.3.3. Result and Discussion 32 1.3.4. Conclusion 46 1.4. Genetic engineering of filamentous bacteriophage (fd) for the application in SERS active probe. 47 1.4.1. Introduction 47 1.4.2. Experimental 48 1.4.3. Result and Discussion 51 1.4.4. Conclusion 59 1.5. References 60 Chapter 2. Development of Sensitivity-Tunable Ion Sensing Platform based on Reverse Electrodialysis 65 2.1. Introduction 65 2.2. Experimental 66 2.3. Result and Discussion 71 2.4. Conclusion 95 2.5. References 96 국문초록 99 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Filamentous Bacteriophage | - |
dc.subject | Bead-based immunoassay | - |
dc.subject | Multiplex analysis | - |
dc.subject | Oxygen evolution reaction catalyst | - |
dc.subject | Reverse electrodialysis | - |
dc.subject | bipolar electrode | - |
dc.subject | ion sensor | - |
dc.subject | sensitivity | - |
dc.subject.ddc | 540 | - |
dc.title | Analytical and Electroanalytical Applications of Filamentous Bacteriophages and Development of Sensitivity-Tunable Ion Sensing Platform based on Reverse Electrodialysis | - |
dc.title.alternative | 섬사상 박테리오파지의 분석∙전기분석적 응용 및 역전기투석을 이용한 민감도 조절 가능 이온 측정 플랫폼의 개발 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.department | 자연과학대학 화학부 | - |
dc.description.degree | Doctor | - |
dc.date.awarded | 2019-08 | - |
dc.contributor.major | 전기분석화학 | - |
dc.identifier.uci | I804:11032-000000156613 | - |
dc.identifier.holdings | 000000000040▲000000000041▲000000156613▲ | - |
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