Association Analysis of Interleukin-1 beta, Interleukin-6, and HMGB1 Variants with Postictal Serum Cytokine Levels in Children with Febrile Seizure and Generalized Epilepsy with Febrile Seizure Plus

Abstract

Background and Purpose Febrile seizure (FS) is a unique type of seizure that only occurs during childhood. Genelized epilepsy with febrile seizure plus (GEFS+) is a familial epilepsy syndrome associated with FS and afebrile seizure (AFS). Both seizure types are related to fever, but whether genetic susceptibility to inflammation is implicated in them is still unclear. To analyze the associations between postictal serum cytokine levels and genetic variants in the cytokine genes interleukin (IL)-1 beta, IL-6, and high mobility group box-1 (HMGB1) in FS and GEFS+. Methods Genotyping was performed in 208 subjects (57 patients with FS, 43 patients with GEFS+, and 108 controls) with the SNaPshot assay for IL-1 beta-31 (rs1143627), IL-1 beta-511 (rs16944), IL-6-572 (rs1800796), and HMGB1 3814 (rs2249825). Serum IL-beta, IL-6, and HMGB1 levels were analyzed within 2 hours after seizure attacks using the ELISA in only 68 patients (38 FS, 10 GEFS+, and 20 controls). The allele distribution, genotype distribution, and correlations with serum cytokine levels were analyzed. Results Near-complete linkage disequilibrium exists between IL-1 beta-31 and IL-1 beta-511 variants. CT genotypes of these variants were associated with significantly higher postictal serum IL-1 beta levels than were CC+TT genotypes in FS (both p<0.05). CT genotypes of IL-beta-31 and IL-1 beta-511 variants were more strongly associated with FS than were CC+TT genotypes (odds ratio=1.691 and 1.731, respectively). For GEFS+, serum IL-1 beta levels after AFS for CT genotypes of IL-beta-31 and IL-1 beta-511 were also higher than for CC+TT genotypes. No significant associations were found for IL-6 and HMGB1. Conclusions Genetic variants located in IL-1 beta-31 and IL-1 beta-511 promotor regions are correlated with higher postictal IL-1 beta levels in FS. These results suggest that IL-1 gene cluster variants in IL-1 beta-31 and IL-1 beta-511 are a host genetic factor for provoking FS in Korean children.