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ETV2/ER71 regulates the generation of FLK1+ cells from mouse embryonic stem cells through miR-126-MAPK signaling

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Authors
Kim, Ju Young; Lee, Dong Hun; Kim, Joo Kyung; Choi, Hong Seo; Dwivedi, Bhakti; Rupji, Manali; Kowalski, Jeanne; Green, Stefan J; Song, Heesang; Park, Won Jong; Chang, Ji Young; Kim, Tae Min; Park, Changwon
Issue Date
2019-11-19
Publisher
BMC
Citation
Stem Cell Research & Therapy, 10(1):328
Keywords
ETV2/ER71FLK1+ cellsmiR-126EGFL7Embryonic stem cells
Abstract
Previous studies including ours have demonstrated a critical function of the transcription factor ETV2 (ets variant 2; also known as ER71) in determining the fate of cardiovascular lineage development. However, the underlying mechanisms of ETV2 function remain largely unknown. In this study, we demonstrated the novel function of the miR (micro RNA)-126-MAPK (mitogen-activated protein kinase) pathway in ETV2-mediated FLK1 (fetal liver kinase 1; also known as VEGFR2)+ cell generation from the mouse embryonic stem cells (mESCs). By performing a series of experiments including miRNA sequencing and ChIP (chromatin immunoprecipitation)-PCR, we found that miR-126 is directly induced by ETV2. Further, we identified that miR-126 can positively regulate the generation of FLK1+ cells by activating the MAPK pathway through targeting SPRED1 (sprouty-related EVH1 domain containing 1). Further, we showed evidence that JUN/FOS activate the enhancer region of FLK1 through AP1 (activator protein 1) binding sequences. Our findings provide insight into the novel molecular mechanisms of ETV2 function in regulating cardiovascular lineage development from mESCs.
ISSN
1757-6512
Language
English
URI
https://doi.org/10.1186/s13287-019-1466-8

http://hdl.handle.net/10371/164752
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College of Medicine/School of Medicine (의과대학/대학원)Pediatrics (소아과학전공)Journal Papers (저널논문_소아과학전공)
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