Publications
Detailed Information
Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells
Cited 38 time in
Web of Science
Cited 46 time in Scopus
- Authors
- Issue Date
- 2005-08
- Publisher
- American Society for Microbiology
- Citation
- Journal of Bacteriology, Vol.187 No.15, pp.5496-5499
- Abstract
- We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.
- ISSN
- 0021-9193
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.