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Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Ryuk-Jun | - |
dc.contributor.author | Kim, Seong Keun | - |
dc.contributor.author | Lee, Seung-Il | - |
dc.contributor.author | Hwang, Su-Jeong | - |
dc.contributor.author | Lee, Gyun Min | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Seol, Wongi | - |
dc.date.accessioned | 2020-04-27T12:30:19Z | - |
dc.date.available | 2020-04-27T12:30:19Z | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.issued | 2006-01 | - |
dc.identifier.citation | Biotechnology Letters, Vol.28 No.1, pp.9-15 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.other | 93228 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165607 | - |
dc.description.abstract | A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells. | - |
dc.language | 영어 | - |
dc.publisher | Kluwer Academic Publishers | - |
dc.title | Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1007/s10529-005-4680-7 | - |
dc.citation.journaltitle | Biotechnology Letters | - |
dc.identifier.wosid | 000234143800002 | - |
dc.identifier.scopusid | 2-s2.0-29244486764 | - |
dc.citation.endpage | 15 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 9 | - |
dc.citation.volume | 28 | - |
dc.identifier.sci | 000234143800002 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BUTYRATE-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | artificial transcription factors | - |
dc.subject.keywordAuthor | Chinese hamster ovary cells | - |
dc.subject.keywordAuthor | recombinant antibody production | - |
dc.subject.keywordAuthor | zinc finger protein | - |
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