Publications

Detailed Information

Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

DC Field Value Language
dc.contributor.authorKwon, Ryuk-Jun-
dc.contributor.authorKim, Seong Keun-
dc.contributor.authorLee, Seung-Il-
dc.contributor.authorHwang, Su-Jeong-
dc.contributor.authorLee, Gyun Min-
dc.contributor.authorKim, Jin-Soo-
dc.contributor.authorSeol, Wongi-
dc.date.accessioned2020-04-27T12:30:19Z-
dc.date.available2020-04-27T12:30:19Z-
dc.date.created2020-03-24-
dc.date.created2020-03-24-
dc.date.created2020-03-24-
dc.date.issued2006-01-
dc.identifier.citationBiotechnology Letters, Vol.28 No.1, pp.9-15-
dc.identifier.issn0141-5492-
dc.identifier.other93228-
dc.identifier.urihttps://hdl.handle.net/10371/165607-
dc.description.abstractA randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.-
dc.language영어-
dc.publisherKluwer Academic Publishers-
dc.titleArtificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells-
dc.typeArticle-
dc.contributor.AlternativeAuthor김진수-
dc.identifier.doi10.1007/s10529-005-4680-7-
dc.citation.journaltitleBiotechnology Letters-
dc.identifier.wosid000234143800002-
dc.identifier.scopusid2-s2.0-29244486764-
dc.citation.endpage15-
dc.citation.number1-
dc.citation.startpage9-
dc.citation.volume28-
dc.identifier.sci000234143800002-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Jin-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusBUTYRATE-INDUCED APOPTOSIS-
dc.subject.keywordPlusMAMMALIAN-CELLS-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusLIBRARIES-
dc.subject.keywordPlusDNA-
dc.subject.keywordAuthorartificial transcription factors-
dc.subject.keywordAuthorChinese hamster ovary cells-
dc.subject.keywordAuthorrecombinant antibody production-
dc.subject.keywordAuthorzinc finger protein-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Related Researcher

  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share