Publications
Detailed Information
Surrogate reporters for enrichment of cells with nuclease-induced mutations
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyojin | - |
dc.contributor.author | Um, Eunji | - |
dc.contributor.author | Cho, Sung-Rae | - |
dc.contributor.author | Jung, Chorong | - |
dc.contributor.author | Kim, Hyongbum | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:34:20Z | - |
dc.date.available | 2020-04-27T12:34:20Z | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.citation | Nature Methods, Vol.8 No.11, pp.941-943 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.other | 93202 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165621 | - |
dc.description.abstract | Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Surrogate reporters for enrichment of cells with nuclease-induced mutations | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1038/NMETH.1733 | - |
dc.citation.journaltitle | Nature Methods | - |
dc.identifier.wosid | 000296891800017 | - |
dc.identifier.scopusid | 2-s2.0-80255134581 | - |
dc.citation.endpage | 943 | - |
dc.citation.number | 11 | - |
dc.citation.startpage | 941 | - |
dc.citation.volume | 8 | - |
dc.identifier.sci | 000296891800017 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.