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TALEN-based knockout library for human microRNAs

Cited 59 time in Web of Science Cited 60 time in Scopus
Authors
Kim, Young-Kook; Wee, Gabbine; Park, Joha; Kim, Jongkyu; Baek, Daehyun; Kim, Jin-Soo; Kim, V. Narry
Issue Date
2013-12
Citation
Nature Structural and Molecular Biology, Vol.20 No.12, pp.1458-1464
Abstract
Various technical tools have been developed to probe the functions of microRNAs (miRNAs), yet their application has been limited by low efficacy and specificity. To overcome the limitations, we used transcription activator-like effector nucleases (TALENs) to knock out human miRNA genes. We designed and produced a library of 540 pairs of TALENs for 274 miRNA loci, focusing on potentially important miRNAs. The knockout procedure takes only 2-4 weeks and can be applied to any cell type. As a case study, we generated knockout cells for two related miRNAs, miR-141 and miR-200c, which belong to the highly conserved miR-200 family. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppress largely nonoverlapping groups of targets, thus suggesting that functional miRNA-target interaction requires strict seed-pairing. Our study illustrates the potency of TALEN technology and provides useful resources for miRNA research.
ISSN
1545-9993
URI
https://hdl.handle.net/10371/165632
DOI
https://doi.org/10.1038/nsmb.2701
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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