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Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koo, Ok Jae | - |
dc.contributor.author | Park, Sol Ji | - |
dc.contributor.author | Lee, Choongil | - |
dc.contributor.author | Kang, Jung Taek | - |
dc.contributor.author | Kim, Sujin | - |
dc.contributor.author | Moon, Joon Ho | - |
dc.contributor.author | Choi, Ji Yei | - |
dc.contributor.author | Kim, Hyojin | - |
dc.contributor.author | Jang, Goo | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Kim, Seokjoong | - |
dc.contributor.author | Lee, Byeong-Chun | - |
dc.date.accessioned | 2020-04-27T12:38:47Z | - |
dc.date.available | 2020-04-27T12:38:47Z | - |
dc.date.created | 2020-03-23 | - |
dc.date.created | 2020-03-23 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.citation | Asian-Australasian journal of animal sciences, Vol.27 No.3, pp.324-329 | - |
dc.identifier.issn | 1011-2367 | - |
dc.identifier.other | 93166 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165636 | - |
dc.description.abstract | To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to create targeted mutations and enriched using a reporter plasmid system. The reporter expresses RFP and eGFP simultaneously when ZFN-mediated site-specific mutations occur. Thus, double positive cells (RFP+/eGFP(+)) were selected and used for somatic cell nuclear transfer. Two types of reporter based enrichment systems were used in this study; the cloned embryos derived from cells enriched using a magnetic sorting-based system showed better developmental competence than did those derived from cells enriched by flow cytometry. Mutated sequences, such as insertions, deletions, or substitutions, together with the wild-type sequence, were found in the cloned porcine blastocysts. Therefore, genetic mutations can be achieved in cloned porcine embryos reconstructed with ZFN-treated cells that were enriched by a reporter-based system. | - |
dc.language | 영어 | - |
dc.publisher | 아세아·태평양축산학회 | - |
dc.title | Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.contributor.AlternativeAuthor | 장구 | - |
dc.contributor.AlternativeAuthor | 이병천 | - |
dc.identifier.doi | 10.5713/ajas.2013.13481 | - |
dc.citation.journaltitle | Asian-Australasian journal of animal sciences | - |
dc.identifier.wosid | 000333141300004 | - |
dc.identifier.scopusid | 2-s2.0-84897692334 | - |
dc.citation.endpage | 329 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 324 | - |
dc.citation.volume | 27 | - |
dc.identifier.sci | 000333141300004 | - |
dc.identifier.kciid | ART001854001 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Jang, Goo | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.contributor.affiliatedAuthor | Lee, Byeong-Chun | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BIALLELIC KNOCKOUT | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PIGS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | NECROSIS | - |
dc.subject.keywordPlus | SWINE | - |
dc.subject.keywordAuthor | Knockout Pig | - |
dc.subject.keywordAuthor | Reporter Based Enrichment | - |
dc.subject.keywordAuthor | Somatic Cell Nuclear Transfer | - |
dc.subject.keywordAuthor | Zinc Finger Nuclease | - |
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