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A guide to genome engineering with programmable nucleases

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dc.contributor.authorKim, Hyongbum-
dc.contributor.authorKim, Jin-Soo-
dc.date.accessioned2020-04-27T12:39:24Z-
dc.date.available2020-04-27T12:39:24Z-
dc.date.created2020-03-23-
dc.date.created2020-03-23-
dc.date.issued2014-05-
dc.identifier.citationNature Reviews Genetics, Vol.15 No.5, pp.321-334-
dc.identifier.issn1471-0056-
dc.identifier.other93169-
dc.identifier.urihttps://hdl.handle.net/10371/165638-
dc.description.abstractProgrammable nucleases - including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system - enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site-specific DNA cleavage in the genome, the repair (through endogenous mechanisms) of which allows high-precision genome editing. However, these nucleases differ in several respects, including their composition, targetable sites, specificities and mutation signatures, among other characteristics. Knowledge of nuclease-specific features, as well as of their pros and cons, is essential for researchers to choose the most appropriate tool for a range of applications.-
dc.language영어-
dc.publisherNature Publishing Group-
dc.titleA guide to genome engineering with programmable nucleases-
dc.typeArticle-
dc.contributor.AlternativeAuthor김진수-
dc.identifier.doi10.1038/nrg3686-
dc.citation.journaltitleNature Reviews Genetics-
dc.identifier.wosid000334599700010-
dc.identifier.scopusid2-s2.0-84898778301-
dc.citation.endpage334-
dc.citation.number5-
dc.citation.startpage321-
dc.citation.volume15-
dc.identifier.sci000334599700010-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Jin-Soo-
dc.type.docTypeReview-
dc.description.journalClass1-
dc.subject.keywordPlusZINC-FINGER NUCLEASES-
dc.subject.keywordPlusTAL EFFECTOR NUCLEASES-
dc.subject.keywordPlusPLURIPOTENT STEM-CELLS-
dc.subject.keywordPlusDNA-BINDING SPECIFICITY-
dc.subject.keywordPlusTARGETED GENE KNOCKOUT-
dc.subject.keywordPlusCRISPR-CAS SYSTEMS-
dc.subject.keywordPlusDOUBLE-STRAND BREAKS-
dc.subject.keywordPlusHIGHLY EFFICIENT-
dc.subject.keywordPlusHOMOLOGOUS RECOMBINATION-
dc.subject.keywordPlusEMBRYO MICROINJECTION-
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  • Department of Chemistry
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