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Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Sangsu | - |
dc.contributor.author | Park, Jeongbin | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:39:59Z | - |
dc.date.available | 2020-04-27T12:39:59Z | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.citation | Bioinformatics, Vol.30 No.10, pp.1473-1475 | - |
dc.identifier.issn | 1367-4803 | - |
dc.identifier.other | 93175 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165640 | - |
dc.description.abstract | The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a targeted manner. CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limited by off-target mutations. Here, we present a novel algorithm termed Cas-OFFinder that searches for potential off-target sites in a given genome or user-defined sequences. Unlike other algorithms currently available for identification of RGEN off-target sites, Cas-OFFinder is not limited by the number of mismatches and allows variations in protospacer-adjacent motif sequences recognized by Cas9, the essential protein component in RGENs. Cas-OFFinder is available as a command-line program or accessible via our website. | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1093/bioinformatics/btu048 | - |
dc.citation.journaltitle | Bioinformatics | - |
dc.identifier.wosid | 000336530000021 | - |
dc.identifier.scopusid | 2-s2.0-84896308706 | - |
dc.citation.endpage | 1475 | - |
dc.citation.number | 10 | - |
dc.citation.startpage | 1473 | - |
dc.citation.volume | 30 | - |
dc.identifier.sci | 000336530000021 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
dc.subject.keywordPlus | HUMAN GENOME | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | SEQUENCES | - |
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