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Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, Young Hoon | - |
dc.contributor.author | Kim, Jong Min | - |
dc.contributor.author | Kim, Hyun-Taek | - |
dc.contributor.author | Lee, Jaehoon | - |
dc.contributor.author | Jeon, Jisun | - |
dc.contributor.author | Jin, Young | - |
dc.contributor.author | Choi, Jung-Hwa | - |
dc.contributor.author | Ban, Young Ho | - |
dc.contributor.author | Ha, Sang-Jun | - |
dc.contributor.author | Kim, Cheol-Hee | - |
dc.contributor.author | Lee, Han-Woong | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:41:40Z | - |
dc.date.available | 2020-04-27T12:41:40Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | Genome Research, Vol.24 No.1, pp.125-131 | - |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.other | 12257 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165645 | - |
dc.description.abstract | RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein: guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F-0 and F-1 mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research. | - |
dc.language | 영어 | - |
dc.publisher | Cold Spring Harbor Laboratory Press | - |
dc.title | Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1101/gr.163394.113 | - |
dc.citation.journaltitle | Genome Research | - |
dc.identifier.wosid | 000329163500012 | - |
dc.identifier.scopusid | 2-s2.0-84891704542 | - |
dc.citation.endpage | 131 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 125 | - |
dc.citation.volume | 24 | - |
dc.identifier.sci | 000329163500012 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
dc.subject.keywordPlus | CRISPR-CAS SYSTEMS | - |
dc.subject.keywordPlus | HUMAN GENOME | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | MUTAGENESIS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | TALENS | - |
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