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Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Witte, Hanh | - |
dc.contributor.author | Moreno, Eduardo | - |
dc.contributor.author | Roedelsperger, Christian | - |
dc.contributor.author | Kim, Jungeun | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Streit, Adrian | - |
dc.contributor.author | Sommer, Ralf J. | - |
dc.date.accessioned | 2020-04-27T12:43:49Z | - |
dc.date.available | 2020-04-27T12:43:49Z | - |
dc.date.created | 2018-11-08 | - |
dc.date.created | 2018-11-08 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | Development Genes and Evolution, Vol.225 No.1, pp.55-62 | - |
dc.identifier.issn | 0949-944X | - |
dc.identifier.other | 67183 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165652 | - |
dc.description.abstract | The diplogastrid nematode Pristionchus pacificus is a nematode model system for comparative studies to Caenorhabditis elegans and integrative evolutionary biology aiming for interdisciplinary approaches of evo-devo, population genetics, and ecology. For this, fieldwork can be combined with laboratory studies, and P. pacificus has a well-developed methodological toolkit of forward genetics, whole genome sequencing, DNA-mediated transformation, and various -omics platforms. Here, we establish CRISPR/Cas9-based gene inactivation and describe various boundary conditions of this methodology for P. pacificus. Specifically, we demonstrate that most mutations arise within the first 9 hours after injections. We systematically tested the efficiency of sgRNAs targeting different exons in Ppa-dpy-1 and characterized the molecular nature of the induced mutations. Finally, we provide a protocol that might also be useful for researchers working with other non-Caenorhabditis nematodes. | - |
dc.language | 영어 | - |
dc.publisher | Springer Verlag | - |
dc.title | Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1007/s00427-014-0486-8 | - |
dc.citation.journaltitle | Development Genes and Evolution | - |
dc.identifier.wosid | 000349029300007 | - |
dc.identifier.scopusid | 2-s2.0-84926669191 | - |
dc.citation.endpage | 62 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 55 | - |
dc.citation.volume | 225 | - |
dc.identifier.sci | 000349029300007 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | CRISPR-CAS9 SYSTEM | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | KNOCKOUT | - |
dc.subject.keywordPlus | REUNION | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordAuthor | Pristionchus pacificus | - |
dc.subject.keywordAuthor | Nematodes | - |
dc.subject.keywordAuthor | CRISPR/Cas9 | - |
dc.subject.keywordAuthor | Gene knockout | - |
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