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Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jeongbin | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Bae, Sangsu | - |
dc.date.accessioned | 2020-04-27T12:46:41Z | - |
dc.date.available | 2020-04-27T12:46:41Z | - |
dc.date.created | 2018-09-19 | - |
dc.date.created | 2018-09-19 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.citation | Bioinformatics, Vol.32 No.13, pp.2017-2023 | - |
dc.identifier.issn | 1367-4803 | - |
dc.identifier.other | 55359 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165662 | - |
dc.description.abstract | Motivation: CRISPR-derived RNA guided endonucleases (RGENs) have been widely used for both gene knockout and knock-in at the level of single or multiple genes. RGENs are now available for forward genetic screens at genome scale, but single guide RNA (sgRNA) selection at this scale is difficult. Results: We develop an online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). With an easy-to-use web interface, Cas-Database allows users to select optimal target sequences simply by changing the filtering conditions. Furthermore, it provides a powerful way to select multiple optimal target sequences from thousands of genes at once for the creation of a genome-wide library. Cas-Database also provides a web application programming interface (web API) for advanced bioinformatics users. | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9 | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1093/bioinformatics/btw103 | - |
dc.citation.journaltitle | Bioinformatics | - |
dc.identifier.wosid | 000379761500013 | - |
dc.identifier.scopusid | 2-s2.0-85007179516 | - |
dc.citation.endpage | 2023 | - |
dc.citation.number | 13 | - |
dc.citation.startpage | 2017 | - |
dc.citation.volume | 32 | - |
dc.identifier.sci | 000379761500013 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | OFF-TARGET SITES | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | FUNCTIONAL GENOMICS | - |
dc.subject.keywordPlus | TOOL | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CRISPR/CAS9 | - |
dc.subject.keywordPlus | NUCLEASES | - |
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