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DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

Cited 198 time in Web of Science Cited 230 time in Scopus
Authors

Baek, Kwangryul; Kim, Duk Hyoung; Jeong, Jooyeon; Sim, Sang Jun; Melis, Anastasios; Kim, Jin-Soo; Jin, EonSeon; Bae, Sangsu

Issue Date
2016-07
Publisher
Nature Publishing Group
Citation
Scientific Reports, Vol.6, p. 30620
Abstract
Microalgae are versatile organisms capable of converting CO2, H2O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9 method rather than plasmids that encode Cas9 and guide RNAs. Outcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutively producing zeaxanthin and showing improved photosynthetic productivity.
ISSN
2045-2322
URI
https://hdl.handle.net/10371/165663
DOI
https://doi.org/10.1038/srep30620
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  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

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