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Fine-Tuning Next-Generation Genome Editing Tools

Cited 40 time in Web of Science Cited 48 time in Scopus
Authors

Kanchiswamy, Chidananda Nagamangala; Maffei, Massimo; Malnoy, Mickael; Velasco, Riccardo; Kim, Jin-Soo

Issue Date
2016-07
Publisher
Elsevier BV
Citation
Trends in Biotechnology, Vol.34 No.7, pp.562-574
Abstract
The availability of genome sequences of numerous organisms and the revolution brought about by genome editing tools (e.g., ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has provided a breakthrough in introducing targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. However, the wider application of these tools in biology, agriculture, medicine, and biotechnology is limited by off-target mutation effects. In this review, we compare available methods for detecting, measuring, and analyzing off-target mutations. Furthermore, we particularly focus on CRISPR/Cas9 regarding various methods, tweaks, and software tools available to nullify off-target effects.
ISSN
0167-7799
URI
https://hdl.handle.net/10371/165665
DOI
https://doi.org/10.1016/j.tibtech.2016.03.007
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  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

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