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Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases

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dc.contributor.authorThomas, Sydney P.-
dc.contributor.authorKim, Eunji-
dc.contributor.authorKim, Jin-Soo-
dc.contributor.authorRaines, Ronald T.-
dc.date.accessioned2020-04-27T12:48:11Z-
dc.date.available2020-04-27T12:48:11Z-
dc.date.issued2016-11-
dc.identifier.citationBiochemistry, Vol.55 No.46, pp.6359-6362-
dc.identifier.issn0006-2960-
dc.identifier.other95214-
dc.identifier.urihttps://hdl.handle.net/10371/165667-
dc.description.abstractRibonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases.-
dc.titleKnockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases-
dc.typeArticle-
dc.contributor.AlternativeAuthor김진수-
dc.identifier.doi10.1021/acs.biochem.6b01003-
dc.citation.journaltitleBiochemistry-
dc.identifier.scopusid2-s2.0-84997820923-
dc.citation.endpage6362-
dc.citation.number46-
dc.citation.startpage6359-
dc.citation.volume55-
dc.identifier.urlhttps://pubs.acs.org/doi/10.1021/acs.biochem.6b01003-
dc.identifier.rimsid95214-
dc.identifier.sci000388913700002-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Jin-Soo-
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