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Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Thomas, Sydney P. | - |
dc.contributor.author | Kim, Eunji | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Raines, Ronald T. | - |
dc.date.accessioned | 2020-04-27T12:48:11Z | - |
dc.date.available | 2020-04-27T12:48:11Z | - |
dc.date.created | 2020-04-08 | - |
dc.date.created | 2020-04-08 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.citation | Biochemistry, Vol.55 No.46, pp.6359-6362 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.other | 95214 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165667 | - |
dc.description.abstract | Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1021/acs.biochem.6b01003 | - |
dc.citation.journaltitle | Biochemistry | - |
dc.identifier.wosid | 000388913700002 | - |
dc.identifier.scopusid | 2-s2.0-84997820923 | - |
dc.citation.endpage | 6362 | - |
dc.citation.number | 46 | - |
dc.citation.startpage | 6359 | - |
dc.citation.volume | 55 | - |
dc.identifier.sci | 000388913700002 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HUMAN PANCREATIC RIBONUCLEASE | - |
dc.subject.keywordPlus | THIOL-DISULFIDE EXCHANGE | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | CONTRIBUTES | - |
dc.subject.keywordPlus | ANGIOGENIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
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