Browse

Myofibroblast in the ligamentum flavum hypertrophic activity

Cited 11 time in Web of Science Cited 15 time in Scopus
Authors
Hur, Junseok W.; Bae, Taegeun; Ye, Sunghyeok; Kim, Joo-Hyun; Lee, Sunhye; Kim, Kyoungmi; Lee, Seung-Hwan; Kim, Jin-Soo; Lee, Jang-Bo; Cho, Tai-Hyoung; Park, Jung-Yul; Hur, Junho K.
Issue Date
2017-08
Citation
European Spine Journal, Vol.26 No.8, pp.2021-2030
Keywords
Ligamentum flavumHypertrophyMyofibroblastsAlpha-smooth muscle actinTransforming growth factor beta1
Abstract
Majority of the previous studies compared lumbar spinal stenosis (LSS) and lumbar disc herniation (LDH) patients for analyses of LFH. However, the separation of normal/hypertrophied LF has often been ambiguous and the severity of hypertrophic activity differed. Here, we present a novel analysis scheme for LFH in which myofibroblast is proposed as a major etiological factor for LFH study. Seventy-one LF patient tissue samples were used for this study. Initially, mRNA levels of the samples were assessed by qRT-PCR: angiopoietin-like protein-2 (ANGPTL2), transforming growth factor-beta1 (TGF-beta 1), vascular endothelial growth factor (VEGF), interleukin-6, collagen-1, 3, 4, 5, and 11, and elastin. Myofibroblasts were detected by immune stain using alpha-smooth muscle actin (alpha SMA) as a marker. To study the myofibroblast in TGF-beta pathway, LF tissues were analyzed for protein levels of alpha SMA/TGF-beta 1 by Western blot. In addition, from LF cells cultured with exogenous TGF-beta 1 conditioned medium, expression of alpha SMA/collagen-1 was assessed and the cell morphology was identified. The comparative analysis of mRNA expression levels (LSS vs LDH) failed to show significant differences in TGF-beta 1 (p = 0.08); however, we found a significant positive correlation among ANGPTL2, VEGF, TGF-beta 1, and collagen-1 and 3, which represent common trends in hypertrophic activity (p < 0.05). We detected myofibroblast in the patient samples by alpha SMA staining, and the protein levels of alpha SMA were positively correlated with TGF-beta 1. In LF cell culture, exogenous TGF-beta 1 upregulated alpha SMA/collagen-1 mRNA levels and facilitated trans-differentiation to myofibroblast. We conclude that the transition of fibroblast to myofibroblasts via TGF-beta pathway is a key linker between inflammation and fibrosis in LFH mechanism.
ISSN
0940-6719
URI
https://hdl.handle.net/10371/165679
DOI
https://doi.org/10.1007/s00586-017-4981-2
Files in This Item:
There are no files associated with this item.
Appears in Collections:
Seoul National University(서울대학교)Featured Researcher's Articles
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse