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Precision genome engineering through adenine base editing in plants

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dc.contributor.authorKang, Beum-Chang-
dc.contributor.authorYun, Jae-Young-
dc.contributor.authorKim, Sang-Tae-
dc.contributor.authorShin, YouJin-
dc.contributor.authorRyu, Jahee-
dc.contributor.authorChoi, Minkyung-
dc.contributor.authorWoo, Je Wook-
dc.contributor.authorKim, Jin-Soo-
dc.date.accessioned2020-04-27T12:58:44Z-
dc.date.available2020-04-27T12:58:44Z-
dc.date.issued2018-07-
dc.identifier.citationNature Plants, Vol.4 No.7, pp.427-431-
dc.identifier.issn2055-026X-
dc.identifier.other80820-
dc.identifier.urihttps://hdl.handle.net/10371/165699-
dc.description.abstractThe recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or missplicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.-
dc.titlePrecision genome engineering through adenine base editing in plants-
dc.typeArticle-
dc.contributor.AlternativeAuthor김진수-
dc.identifier.doi10.1038/s41477-018-0178-x-
dc.citation.journaltitleNature Plants-
dc.identifier.scopusid2-s2.0-85048029625-
dc.citation.endpage431-
dc.citation.number7-
dc.citation.startpage427-
dc.citation.volume4-
dc.identifier.urlhttps://www.nature.com/articles/s41477-018-0178-x-
dc.identifier.rimsid80820-
dc.identifier.sci000443221200014-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Jin-Soo-
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