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Transcriptomic analysis of genes modulated by cyclo(l-phenylalanine-l-proline) in vibrio vulnificus

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors
Kim, In Hwang; Son, Jee-Soo; Wen, Yancheng; Jeong, Sang-Min; Min, Ga-Young; Park, Na-Young; Lee, Keun-Woo; Cho, Yong-Joon; Chun, Jongsik; Kim, Kun-Soo
Issue Date
2013-12
Citation
Journal of Microbiology and Biotechnology, Vol.23 No.12, pp.1791-1801
Keywords
Vibrio vulnificuscyclo(Phe-Pro)next-generation sequencingtranscriptomebiofilm
Abstract
Diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. Vibrio vulnificus, which causes septicemia in humans, produces cyclo(L-phenylalanine-L-proline) (cFP). To understand the biological roles of cFP, the effect of the compound on the expression of the total mRNA in V. vulnificus was assessed by next-generation sequencing. Based on the transcriptomic analysis, we classified the cFP-regulated genes into functional categories and clustered them according to the expression patterns resulted from treatment with cFP. From a total of 4,673 genes, excepting the genes encoding tRNA in V. vulnificus, 356 genes were up-regulated and 602 genes were down-regulated with an RPKM (reads per kilobase per million) value above 3. The genes most highly induced by cFP comprised those associated with the transport and metabolism of inorganic molecules, particularly iron. The genes negatively regulated by cFP included those associated with energy production and conversion, as well as carbohydrate metabolism. Noticeably, numerous genes related with biofilm formation were modulated by cFP. We demonstrated that cFP interferes significantly with the biofilm formation of V. vulnificus.
ISSN
1017-7825
URI
http://hdl.handle.net/10371/165806
DOI
https://doi.org/10.4014/jmb.1308.08068
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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