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Simplified EM grid vitrification is a convenient and efficient method for mouse mature oocyte cryopreservation

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dc.contributor.authorKim, Seok Hyun-
dc.contributor.authorKu, Seung-Yup-
dc.contributor.authorSung, Ki Cheong-
dc.contributor.authorKang, Moon Joo-
dc.contributor.authorKim, Sung Ah-
dc.contributor.authorKim, Hee Sun-
dc.contributor.authorOh, Sun Kyung-
dc.contributor.authorJee, Byung Chul-
dc.contributor.authorSuh, Chang Suk-
dc.contributor.authorChoi, Young Min-
dc.contributor.authorKim, Jung Gu-
dc.contributor.authorMoon, Shin Yong-
dc.date.accessioned2009-11-27T05:02:02Z-
dc.date.available2009-11-27T05:02:02Z-
dc.date.issued2006-06-30-
dc.identifier.citationYonsei Med J. 2006 Jun 30;47(3):399-404.en
dc.identifier.issn0513-5796 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16807991-
dc.identifier.urihttps://hdl.handle.net/10371/16597-
dc.description.abstractThis study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compared with those of the slow freezing method using 1.5M DMSO. Then, conventional EM grid vitrification was compared with simplified EM grid vitrification. Simplified EM grid vitrification showed higher survival, fertilization and hatching rates than those of the slow freezing method (85.6% vs. 63.2%; 51.0% vs. 22.3%; 38.7% vs. 12.5%, p < 0.01, respectively). Moreover, simplified EM grid vitrification showed higher recovery, survival and fertilization rates than those of conventional EM grid vitrification (100% vs. 95.0%, p=0.024; 90.0% vs. 78.9%, p=0.033; 56.7% vs. 38.7%, p=0.021, respectively). Hatching rate tended to be higher for simplified EM grid vitrification compared to conventional EM grid vitrification (41.1% vs. 24.1%). In conclusion, simplified EM grid vitrification is a convenient and efficient method for cryopreservation of mouse mature oocytes, compared to conventional EM grid vitrification and slow freezing methods.en
dc.language.isoen-
dc.publisherYonsei University College of Medicineen
dc.subjectAnimalsen
dc.subjectCell Survivalen
dc.subjectCryopreservation/*instrumentation/*methodsen
dc.subjectFemaleen
dc.subject*Fertilization in Vitroen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred C57BLen
dc.subjectMice, Inbred DBAen
dc.subjectOocytes/*cytologyen
dc.subjectPregnancyen
dc.titleSimplified EM grid vitrification is a convenient and efficient method for mouse mature oocyte cryopreservationen
dc.typeArticleen
dc.contributor.AlternativeAuthor김석현-
dc.contributor.AlternativeAuthor구승엽-
dc.contributor.AlternativeAuthor성기청-
dc.contributor.AlternativeAuthor강문주-
dc.contributor.AlternativeAuthor김성아-
dc.contributor.AlternativeAuthor김희선-
dc.contributor.AlternativeAuthor오선경-
dc.contributor.AlternativeAuthor지병철-
dc.contributor.AlternativeAuthor서창석-
dc.contributor.AlternativeAuthor최영민-
dc.contributor.AlternativeAuthor김정구-
dc.contributor.AlternativeAuthor문신용-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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