Nocardioides dokdonensis sp. nov., an actinomycete isolated from sand sediment

Abstract

A strictly aerobic, non-motile, short rod-shaped actinomycete, designated strain FR1436(T), was isolated from sand sediment from a beach on Dokdo Island, Republic of Korea. The major menaquinone was MK-8(H(4)), the predominant cellular fatty acid was iso-C(16:0) (40.4 %) and the G + C content of the DNA was 69.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain FR1436(T) formed a lineage within the genus Nocardioides and was closely related to Nocardioides marinisabuli SBS-12(T) (97.8% sequence similarity) and Nocardioides salarius CL-Z59(T) (97.4%). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain FR1436(T) from N. marinisabuli. On the basis of the evidence presented in this study, strain FR1436(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides dokdonensis sp. nov. is proposed. The type strain is FR1436(T) (=KCTC 19309(T) = JCM 14815(T)).