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EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences

Cited 1758 time in Web of Science Cited 1827 time in Scopus
Authors
Chun, Jongsik; Lee, Jae-Hak; Jung, Yoonyoung; Kim, Myungjin; Kim, Seil; Kim, Byung Kwon; Lim, Young-Woon
Issue Date
2007-10
Citation
International Journal of Systematic and Evolutionary Microbiology, Vol.57, pp.2259-2261
Abstract
16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleoticle similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates.
ISSN
1466-5026
URI
https://hdl.handle.net/10371/166003
DOI
https://doi.org/10.1099/ijs.0.64915-0
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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