Glaciecola nitratireducens sp. nov., isolated from seawater

Abstract

A marine bacterial strain, FR1064(T), was isolated from a surface seawater sample collected off Jeju Island, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the Gammaproteobacteria and was related to the genus Glaciecola with 97 center dot 6 % sequence similarity to Glaciecola pallidula, its nearest phylogenetic neighbour. DNA-DNA relatedness between strain FR1064(T) and G. pallidula ACAM 615(T) was 55 %. Cells of the novel isolate were Gram-negative, aerobic, rod-shaped, motile and halophilic, with an optimum sea salts concentration of 4-7 %. The major fatty acids were straight-chain saturated (C16: 0), summed feature 3 and monounsaturated fatty acid C-18:1. The DNA G + C content was 44 mol%. Several phenotypic characteristics differentiated the novel isolate from all previously described members of the genus Glaciecola. The polyphasic data obtained in this study clearly demonstrate that strain FR1064(T) represents a novel species of the genus Glaciecola. The name Glaciecola nitratireducens sp. nov. is therefore proposed, with strain FR1064(T) (=KCTC 12276(T)=JCM 12485(T)) as the type strain.