Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea

Abstract

Two actinomycete strains, DSA1(T) and HD9(T), were isolated from a potato tuber and soil from a potato-cultivating field in Jeju, Korea, respectively. A comprehensive 16S rDNA analysis revealed that the isolates belong to the genus Kribbella and share 97-7-98-6% sequence similarity to Kribbella species. The strains also contained typical chemotaxonomic markers of the genus Kribbella: LL-diaminopimelic acid, alanine, glycine and glutamic acid in the cell wall peptidoglycan; mannose, glucose, galactose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as characteristic phospholipids; and the major menaquinone MK-9 (H-4). DNA-DNA hybridization experiments showed that the isolates represented two distinct genomic species. A number of phenotypic properties can be used to differentiate the two isolates from Kribbella species. On the basis of polyphasic evidence, two novel species are proposed: Kribbella solani sp. nov. for strain DSA1(T) (= KACC 20196(T)=JCM 12205(T)) and Kribbella jejuensis sp. nov. for strain HD9(T) (=KACC 20266 (T)=JCM 12204(T)).