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Nocardia crassostreae sp, nov., the causal agent of nocardiosis in Pacific oysters

Cited 59 time in Web of Science Cited 64 time in Scopus
Authors

Friedman, Carolyn S.; Beaman, Blaine L.; Chun, Jongsik; Goodfellow, Michael; Gee, Arthur; Hedrick, Ronald P.

Issue Date
1998-01
Publisher
Iowa State University Press
Citation
International Journal of Systematic Bacteriology, Vol.48, pp.237-246
Abstract
Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso-diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G+C-rich DNA, All of these properties are consistent with the classification of the organisms in the genus Nocardia. A partial sequence of the 16S rRNA gene of isolate NB4H was determined following isolation and cloning of the PCR-amplified gene. The sequence was aligned with those of representative mycolic-acid-containing taxa and a phylogenetic tree was generated using the neighbour-joining method, It was evident from the phylogenetic tree that the three strains tested, RB1, OB3P and NB4H, were identical and belonged to the Nocardia otitidiscaviarum rRNA sub-group, The biochemical, chemical, morphological and physiological properties of the isolates were also essentially identical and served to distinguish them from representative nocardiae, It is, therefore, proposed that the strains isolated from the diseased Pacific oysters be assigned to a new species, Nocardia crassostreae, The type strain is NB4H (= ATCC 700418).
ISSN
0020-7713
URI
https://hdl.handle.net/10371/166193
DOI
https://doi.org/10.1099/00207713-48-1-237
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