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Antimicrobial Metabolites from Streptomyces bacillaris MBTH40 : 방선균 Streptomyces bacillaris MBTH40 대사산물의 항균활성 연구

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dc.contributor.advisor오기봉-
dc.contributor.author강새연-
dc.date.accessioned2020-05-07T04:06:51Z-
dc.date.available2020-05-07T04:06:51Z-
dc.date.issued2020-
dc.identifier.other000000158566-
dc.identifier.urihttp://dcollection.snu.ac.kr/common/orgView/000000158566ko_KR
dc.description학위논문(석사)--서울대학교 대학원 :농업생명과학대학 농생명공학부,2020. 2. 오기봉.-
dc.description.abstractActinobacteria is an attractive phylum to produce diverse secondary metabolites with high utility as a source of anti-infectives. Marine-derived actinobacteria is relatively unrevealed realm due to the low accessibility. Since the factors including pressure, light, temperature and concentration of oxygen are extremely various compared to the soil environment, marine actinobacteria are expected to produce metabolites with novel structures and mode of actions. The sediment of the deep sea located in the Pacific Ocean was provided by Korea Institute of Ocean Science & Technology (KIOST). In this study, 406 actinobacteria isolates from the sediment were screened and Streptomyces bacillaris MBTH40 was selected guided by the inhibitory activity against Staphylococcus aureus. Through subsequent extraction and separation, four compounds were isolated, and the structures were elucidated via spectroscopic analyses. All the compounds were revealed as known materials. Afterwards, antibacterial, antifungal and isocitrate lyase (ICL) inhibition activities of each compound were investigated. The results present notable inhibitory activity of lactoquinomycin A against pathogenic bacteria including MRSA strains. None of the compounds exhibited antifungal activity, while lactoquinomycin B was found to moderately inhibit activity of ICL.-
dc.description.abstract방선균은 약리학적 가치를 갖는 다양한 이차 대사산물을 생산하며 인간에게 유용한 물질들을 제공하여 왔다. 특히 상대적으로 연구가 덜 이루어진 해양 방선균은 다양한 압력, 온도, 빛과 산소 조건 등 토양과는 다른 환경 조건에 서식하기에 이에 기인한 특성의 일원으로 신물질 생산 대상으로서 주목받고 있다. 한국해양과학기술원으로부터 제공받은 태평양 해저 토양으로부터 분리한 406종의 방선균 중 Staphylococcus aureus 라는 병원균에 저해 활성을 보이는 40H를 선정하였다. 이후 16S ribosomal RNA 유전자 분석을 통하여 Streptomyces bacillaris 로 동정하였고 균주 이름을 MBTH40으로 명명하였다. 액체-액체 추출 및 HPLC를 통하여 대사산물을 분리하였고 분광학적 데이터를 얻어 총 네 가지 물질의 구조를 규명하였다. 모든 물질들은 이미 발견된 화합물들이었다. 각 물질의 항세균, 항진균, 그리고 isocitrate lyase (ICL) 효소 저해 활성여부를 실험한 결과 lactoquinomycin A가 methicillin-resistant Staphylococcus aureus (MRSA) 균주를 포함한 병원성 세균에 대하여 저해활성을 가장 강하게 나타냈다. 항진균 활성을 나타낸 물질은 없었으며 lactoquinomycin B 가 약하게 ICL의 활성을 저해하는 것으로 나타났다.-
dc.description.tableofcontentsIntroduction 1

Materials and Methods 6
Screening of marine-derived actinobacteria 6
Identification of Streptomyces bacillaris MBTH40 7
Cultivation of S. bacillaris MBTH40 7
Extraction and separation of metabolites from strain MBTH40 culture 8
Purification of the compounds 9
Structure elucidation 9
Antibacterial activity assay 10
Antifungal activity assay 10
ICL inhibition assay 11

Results 13
Selection and cultivation of 40H 13
Identification of S. bacillaris MBTH40 13
Isolation of the compounds 20
Structure elucidation 27
Antibacterial and antifungal activity test 36
ICL inhibitory assay 40

Discussion 42

References 46
Supplementary Materials 49
Appendix 70
Abstract in Korean 71
Acknowledgements 73
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dc.language.isoeng-
dc.publisher서울대학교 대학원-
dc.subject.ddc630-
dc.titleAntimicrobial Metabolites from Streptomyces bacillaris MBTH40-
dc.title.alternative방선균 Streptomyces bacillaris MBTH40 대사산물의 항균활성 연구-
dc.typeThesis-
dc.typeDissertation-
dc.contributor.AlternativeAuthorKang, Sae Yeon-
dc.contributor.department농업생명과학대학 농생명공학부-
dc.description.degreeMaster-
dc.date.awarded2020-02-
dc.contributor.major응용생명화학전공-
dc.identifier.uciI804:11032-000000158566-
dc.identifier.holdings000000000042▲000000000044▲000000158566▲-
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