Publications
Detailed Information
NAD(P)-dependent steroid dehydrogenase-like is involved in breast cancer cell growth and metastasis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, So-Hyun | - |
dc.contributor.author | Kim, Hoe Suk | - |
dc.contributor.author | Kim, Ryong Nam | - |
dc.contributor.author | Jung, So-Youn | - |
dc.contributor.author | Hong, Bok Sil | - |
dc.contributor.author | Kang, Eun Ji | - |
dc.contributor.author | Lee, Han-Byoel | - |
dc.contributor.author | Moon, Hyeong-Gon | - |
dc.contributor.author | Noh, Dong-Young | - |
dc.contributor.author | Han, Wonshik | - |
dc.date.accessioned | 2020-08-11T07:29:38Z | - |
dc.date.available | 2020-08-11T07:29:38Z | - |
dc.date.issued | 2020-05-04 | - |
dc.identifier.citation | BMC Cancer, 20(1):375 | ko_KR |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | https://hdl.handle.net/10371/168702 | - |
dc.description.abstract | The cholesterol biosynthesis pathway is typically upregulated in breast cancer. The role of NAD(P)-dependent steroid dehydrogenase-like (NSDHL) gene, which is involved in cholesterol biosynthesis, in breast cancer remains unknown. This study aimed to uncover the role of NSDHL in the growth and metastasis of breast cancer.
After NSDHL knockdown by transfection of short interfering RNA into human breast cancer cell lines (MCF-7, MDA-MB-231 and BT-20) and human breast epithelial cell line (MCF10A), cell proliferation assay, cell cycle analysis, three-dimensional cell culture, clonogenic assay, transwell migration and invasion assays, and wound healing assay were performed. Erlotinib was used as the target drug for epidermal growth factor receptor. Immunodeficient mice (NOD.Cg-Prkdcscid Il2rgtm1wjl /SzJ) were used as orthotropic breast tumor models by injecting them with NSDHL-knockdown MDA-MB-231 cells using lentivirus-carrying NSDHL short hairpin RNA. Clinical data from 3951 breast cancer patients in Gene Expression Omnibus databases were used to investigate the potential prognostic role of NSDHL by survival analysis. NSDHL knockdown in BT-20, and MDA-MB-231 resulted in a significant decrease in their viability, colony formation, migration, and invasion abilities (p < 0.05). Total cholesterol levels were observed to be significantly decreased in NSDHL-knockdown BT-20 and MDA-MB-231 (p < 0.0001). NSDHL knockdown significantly increased the rate of erlotinib-induced cell death, especially in MDA-MB-231 (p = 0.01). NSDHL knockdown led to significantly decreased tumor growth and lung metastasis in the MDA-MB-231 xenograft model (p < 0.01). Clinically, high NSDHL expression in tumors of patients with breast cancer was associated with significantly reduced recurrence-free survival (p < 0.0001). NSDHL might have a role in promoting breast cancer progression. The usage of NSDHL as a therapeutic target in breast cancer needs to be clarified in further studies. | ko_KR |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea
(NRF) grant funded by the Korea government (MSIT) (No. 2018R1A2A2A05022732). The funding body had no role in the design of the study and collection, analysis, and interpretation of data, nor in writing the manuscript | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Breast cancer | - |
dc.subject | NSDHL | - |
dc.subject | Knockdown | - |
dc.subject | Proliferation | - |
dc.subject | Metastasis | - |
dc.subject | Cholesterol | - |
dc.subject | EGF | - |
dc.title | NAD(P)-dependent steroid dehydrogenase-like is involved in breast cancer cell growth and metastasis | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 윤소현 | - |
dc.contributor.AlternativeAuthor | 김회석 | - |
dc.contributor.AlternativeAuthor | 김룡남 | - |
dc.contributor.AlternativeAuthor | 정소윤 | - |
dc.contributor.AlternativeAuthor | 홍복실 | - |
dc.contributor.AlternativeAuthor | 강은지 | - |
dc.contributor.AlternativeAuthor | 이한별 | - |
dc.contributor.AlternativeAuthor | 문형곤 | - |
dc.contributor.AlternativeAuthor | 노동영 | - |
dc.contributor.AlternativeAuthor | 한원식 | - |
dc.identifier.doi | 10.1186/s12885-020-06840-2 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2020-06-17T13:01:53Z | - |
dc.citation.endpage | 388 | ko_KR |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 375 | ko_KR |
dc.citation.volume | 20 | ko_KR |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.