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Studies on synaptic engram in hippocampus and amygdala using Dual-eGRASP : Dual-eGRASP 기술을 이용한 해마 및 편도에서의 기억 저장 시냅스 연구

DC Field Value Language
dc.contributor.advisor강봉균-
dc.contributor.author최동일-
dc.date.accessioned2020-10-13T04:05:25Z-
dc.date.available2020-10-13T04:05:25Z-
dc.date.issued2020-
dc.identifier.other000000162575-
dc.identifier.urihttps://hdl.handle.net/10371/170765-
dc.identifier.urihttp://dcollection.snu.ac.kr/common/orgView/000000162575ko_KR
dc.description학위논문 (박사) -- 서울대학교 대학원 : 자연과학대학 협동과정 뇌과학전공, 2020. 8. 강봉균.-
dc.description.abstractOur daily experiences are stored in the brain as form of memories and define one person's individuality. It has long been human beings interests, where and how the memory is encoded in our brain. In this acquisition and storage process of memories, structural and physiological changes occur, which is considered as the physical substrates of the memory or the engram. During the memory formation, synapses between neurons undergo plasticity. Neurobiologists have tried to observe these changes in the synapses between engram cells. However, substrate at synapse-level within engram cells remains elusive, because of technical limitations. To distinguish the connections between engram cell and other neurons, I developed and applied dual-eGRASP technique.
Using Dual-eGRASP, I could capture the Synaptic engrams in the hippocampus and amygdala, which are the connections between pre- and post-synaptic engram cells among intermingled neuronal ensembles. Tracking the synaptic engram in hippocampus CA3 to CA1 Schaffer-collateral pathway, I found the strength of memory is correlated with density and spine size of synaptic engrams, not depend on number of engram cells. Moreover, in the lateral amygdala (LA), I found that extinction of fear memory reversed the synaptic enhancement induced by fear conditioning. In addition, re-conditioning with same tone and shock recovered the spine size of synaptic engram decreased by fear extinction. These results indicate that the connections between engram cells, synaptic engram, represents the state of memory. These synaptic-level engram approaches will provide information on the question, how the memory is encoded in our brain.
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dc.description.tableofcontentsChapter I. Introduction 7
Background 8
Purpose of this study 15
Chapter II. Identification of synaptic engram using DualeGRASP 17
Introduction 18
Experimental Procedures 20
Results 25
Discussion 36
Chapter III. Strength of memory is correlated with density and spine size of synaptic engram 38
Introduction 39
Experimental Procedures 41
Results 46
Discussion 58
Chapter IV. Synaptic engram represents the state of memory; Conditioning, Extinction, Re-conditioning 59
Introduction 60
Experimental Procedures 62
Results 67
Discussion 79
Chapter V. Conclusion 82
References 85
국문초록 90
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dc.language.isoeng-
dc.publisher서울대학교 대학원-
dc.subjectMemory-
dc.subjectEngram-
dc.subjectHippocampus-
dc.subjectAmygdala-
dc.subjectExtinction-
dc.subjectDual-eGRASP-
dc.subject기억-
dc.subject엔그램-
dc.subject해마-
dc.subject편도-
dc.subject기억소거-
dc.subject.ddc611.81-
dc.titleStudies on synaptic engram in hippocampus and amygdala using Dual-eGRASP-
dc.title.alternativeDual-eGRASP 기술을 이용한 해마 및 편도에서의 기억 저장 시냅스 연구-
dc.typeThesis-
dc.typeDissertation-
dc.contributor.department자연과학대학 협동과정 뇌과학전공-
dc.description.degreeDoctor-
dc.date.awarded2020-08-
dc.identifier.uciI804:11032-000000162575-
dc.identifier.holdings000000000043▲000000000048▲000000162575▲-
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