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Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory amino acid carrier 1 expression and oxidative stress in SH-SY5Y cells and the hippocampus of mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Han-Byeol | - |
dc.contributor.author | Yoo, Ji-Young | - |
dc.contributor.author | Yoo, Seung-Yeon | - |
dc.contributor.author | Lee, Jun-Ho | - |
dc.contributor.author | Chang, Wonseok | - |
dc.contributor.author | Kim, Hye-Sun | - |
dc.contributor.author | Baik, Tai-Kyoung | - |
dc.contributor.author | Woo, Ran-Sook | - |
dc.date.accessioned | 2021-01-19T06:16:51Z | - |
dc.date.available | 2021-01-19T15:22:56Z | - |
dc.date.issued | 2020-11-13 | - |
dc.identifier.citation | Molecular Brain. 2020 Nov 13;13(1):153 | ko_KR |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171659 | - |
dc.description.abstract | Excitatory amino acid carrier 1 (EAAC1) is an important subtype of excitatory amino acid transporters (EAATs) and is the route for neuronal cysteine uptake. CoCl2 is not only a hypoxia-mimetic reagent but also an oxidative stress inducer. Here, we found that CoCl2 induced significant EAAC1 overexpression in SH-SY5Y cells and the hippocampus of mice. Transient transfection of EAAC1 reduced CoCl2-induced cytotoxicity in SH-SY5Y cells. Based on this result, upregulation of EAAC1 expression by CoCl2 is thought to represent a compensatory response against oxidative stress in an acute hypoxic state. We further demonstrated that pretreatment with Neuregulin-1 (NRG1) rescued CoCl2-induced upregulation of EAAC1 and tau expression. NRG1 plays a protective role in the CoCl2-induced accumulation of reactive oxygen species (ROS) and reduction in antioxidative enzyme (SOD and GPx) activity. Moreover, NRG1 attenuated CoCl2-induced apoptosis and cell death. NRG1 inhibited the CoCl2-induced release of cleaved caspase-3 and reduction in Bcl-XL levels. Our novel finding suggests that NRG1 may play a protective role in hypoxia through the inhibition of oxidative stress and thereby maintain normal EAAC1 expression levels. | ko_KR |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science and Technology (NRF2019R1H1A2079060 awarded to RSW, NRF-2020R1A2C1011839 awarded to HSK and 2017R1D1A1B03028729 awarded to JHL). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | CoCl2 | - |
dc.subject | EAAC1 | - |
dc.subject | Neuregulin-1 | - |
dc.subject | Oxidative stress | - |
dc.subject | Apoptosis | - |
dc.title | Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory amino acid carrier 1 expression and oxidative stress in SH-SY5Y cells and the hippocampus of mice | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김한별 | - |
dc.contributor.AlternativeAuthor | 유지영 | - |
dc.contributor.AlternativeAuthor | 유승연 | - |
dc.contributor.AlternativeAuthor | 이준호 | - |
dc.contributor.AlternativeAuthor | 장원석 | - |
dc.contributor.AlternativeAuthor | 김혜선 | - |
dc.contributor.AlternativeAuthor | 백태경 | - |
dc.contributor.AlternativeAuthor | 우난숙 | - |
dc.identifier.doi | 10.1186/s13041-020-00686-2 | - |
dc.citation.journaltitle | Molecular Brain | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2020-11-15T04:08:55Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 153 | ko_KR |
dc.citation.volume | 13 | ko_KR |
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