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Temporal landscape of microRNA-mediated host-virus crosstalk during productive human cytomegalovirus infection

Cited 30 time in Web of Science Cited 32 time in Scopus
Authors
Kim, Sungchul; Seo, Daekwan; Kim, Dongwoo; Hong, Yujin; Chang, Hyeshik; Baek, Daehyun; Kim, V. Narry; Lee, Sungwook; Ahn, Kwangseog
Issue Date
2015-06
Citation
Cell Host and Microbe, Vol.17 No.6, pp.838-851
Abstract
Temporal profiles of miRNA activity during productive virus infection can provide fundamental insights into host-virus interactions. Most reported miRNA targetome analyses in the context of virus infection have been performed in latently infected cells and lack reliable models for quantifying the suppression efficacy at specific miRNA target sites. Here, we identified highly competent temporal miRNA targetomes during lytic HCMV infection by using AGO-CLIP-seq together with a bioinformatic method that quantifies miRNA functionality at a specific target site, called ACE-scoring. The repression efficiency at target sites correlates with the magnitude of the ACE-score, and temporal HCMV-encoded miRNA targetomes identified by ACE-scoring were significantly enriched in functional categories involved in pathways central for HCMV biology. Furthermore, comparative analysis between human and viral miRNA targetomes supports the existence of intimate cooperation and co-targeting between them. Our holistic survey provides a valuable resource for understanding host-virus interactions during lytic HCMV infection.
ISSN
1931-3128
URI
https://hdl.handle.net/10371/171869
DOI
https://doi.org/10.1016/j.chom.2015.05.014
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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