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microRNA-directed cleavage of ATHB15 mRNA regulates vascular development in Arabidopsis inflorescence stems

Cited 250 time in Web of Science Cited 253 time in Scopus
Authors
Kim, Joonki; Jung, Jae‐Hoon; Reyes, Jose L.; Kim, Youn‐Sung; Kim, Sun‐Young; Chung, Kyung‐Sook; Kim, Jin A.; Lee, Minsun; Lee, Yoontae; Kim, V. Narry; Chua, Nam‐Hai; Park, Chung-Mo
Issue Date
2005-04
Citation
Plant Journal, Vol.42 No.1, pp.84-94
Keywords
ArabidopsisATHB15HD-ZIPmicroRNAmRNA cleavagevascular development
Abstract
Class III homeodomain-leucine zipper proteins regulate critical aspects of plant development, including lateral organ polarity, apical and lateral meristem formation, and vascular development. ATHB15, a member of this transcription factor family, is exclusively expressed in vascular tissues. Recently, a microRNA (miRNA) binding sequence has been identified in ATHB15 mRNA, suggesting that a molecular mechanism governed by miRNA binding may direct vascular development through ATHB15. Here, we show that miR166-mediated ATHB15 mRNA cleavage is a principal mechanism for the regulation of vascular development. In a gain-of-function MIR166a mutant, the decreased transcript level of ATHB15 was accompanied by an altered vascular system with expanded xylem tissue and interfascicular region, indicative of accelerated vascular cell differentiation from cambial/procambial cells. A similar phenotype was observed in Arabidopsis plants with reduced ATHB15 expression but reversed in transgenic plants overexpressing an miR166-resistant ATHB15. ATHB15 mRNA cleavage occurred in standard wheat germ extracts and in Arabidopsis and was mediated by miR166 in Nicotiana benthamiana cells. miR166-assisted ATHB15 repression is likely to be a conserved mechanism that regulates vascular development in all vascular plants.
ISSN
0960-7412
URI
https://hdl.handle.net/10371/171876
DOI
https://doi.org/10.1111/j.1365-313X.2005.02354.x
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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