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Processing of intronic microRNAs

Cited 516 time in Web of Science Cited 539 time in Scopus
Authors
Kim, Young-Kook; Kim, V. Narry
Issue Date
2007-02
Citation
EMBO Journal, Vol.26 No.3, pp.775-783
Keywords
DroshaintronMicroRNAprocessingsplicing
Abstract
The majority of human microRNA ( miRNA) loci are located within intronic regions and are transcribed by RNA polymerase II as part of their hosting transcription units. The primary transcripts are cleaved by Drosha to release similar to 70 nt pre-miRNAs that are subsequently processed by Dicer to generate mature similar to 22 nt miRNAs. It is generally believed that intronic miRNAs are released by Drosha from excised introns after the splicing reaction has occurred. However, our database searches and experiments indicate that intronic miRNAs can be processed from unspliced intronic regions before splicing catalysis. Intriguingly, cleavage of an intron by Drosha does not significantly affect the production of mature mRNA, suggesting that a continuous intron may not be required for splicing and that the exons may be tethered to each other. Hence, Drosha may cleave intronic miRNAs between the splicing commitment step and the excision step, thereby ensuring both miRNA biogenesis and protein synthesis from a single primary transcript. Our study provides a novel example of eukaryotic gene organization and RNA-processing control.
ISSN
0261-4189
URI
http://hdl.handle.net/10371/171878
DOI
https://doi.org/10.1038/sj.emboj.7601512
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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