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The Y14 protein communicates to the cytoplasm the position of exon-exon junctions

Cited 164 time in Web of Science Cited 165 time in Scopus
Issue Date
2001-04
Citation
EMBO Journal, Vol.20 No.8, pp.2062-2068
Keywords
AlymRNAnuclear transportsplicingY14
Abstract
We recently described an RNA-binding protein, Y14, that binds preferentially to spliced mRNAs and persists in the cytoplasm, Y14 is part of a multi-protein complex that also contains the mRNA export factor TAP. This suggests that splicing imprints the mRNA with a unique set of proteins that communicate the history of the transcript to the cytoplasm, Here, using microinjection of pre-mRNAs into Xenopus oocyte nuclei followed by immunoprecipitation of RNase-fragmented mRNAs from the cytoplasm, we show that Y14 is stably bound to sequences immediately upstream of exon-exon junctions. This feature appears to be unique to Y14, Using monoclonal antibodies that we produced against Aly/REF, another component recently reported to be an mRNA export factor, we show that Aly/REF is associated with spliced mRNAs in the nucleus but is not detectable on mRNAs in the cytoplasm, Thus, we propose that the splicing-dependent binding of Y14 provides a position-specific molecular memory that communicates to the cytoplasm the location of exon and intron boundaries. This novel mechanism may play an important role in post-splicing events.
ISSN
0261-4189
URI
https://hdl.handle.net/10371/171887
DOI
https://doi.org/10.1093/emboj/20.8.2062
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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