Browse

Role of the small subunit processome in the maintenance of pluripotent stem cells

Cited 43 time in Web of Science Cited 45 time in Scopus
Authors
You, Kwon Tae; Park, Joha; Kim, V. Narry
Issue Date
2015-10
Citation
Genes and Development, Vol.29 No.19, pp.2004-2009
Keywords
RNA-binding proteinembryonic stem cellSSU processomepluripotencytranslational controlribosome
Abstract
RNA-binding proteins (RBPs) play integral roles in gene regulation, yet only a small fraction of RBPs has been studied in the context of stem cells. Here we applied an RNAi screen for RBPs in mouse embryonic stem cells (ESCs) and identified 16 RBPs involved in pluripotency maintenance. Interestingly, six identified RBPs, including Krr1 and Ddx47, are part of a complex called small subunit proces-some (SSUP) that mediates 18S rRNA biogenesis. The SSUP components are preferentially expressed in stem cells and enhance the global translational rate, which is critical to sustain the protein levels of labile pluripotency factors such as Nanog and Esrrb. Furthermore, the SSUP proteins are required for efficient reprogramming of induced pluripotent stem cells. Our study uncovers the role of the SSUP and the importance of translational control in stem cell fate decision.
ISSN
0890-9369
URI
https://hdl.handle.net/10371/171895
DOI
https://doi.org/10.1101/gad.267112.115
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse