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mTAIL-seq reveals dynamic poly(A) tail regulation in oocyte-to-embryo development

Cited 57 time in Web of Science Cited 58 time in Scopus
Authors
Lim, Jaechul; Lee, Mihye; Son, Ahyeon; Chang, Hyeshik; Kim, V. Narry
Issue Date
2016-07
Citation
Genes and Development, Vol.30 No.14, pp.1671-1682
Keywords
poly(A) tailTAIL-seqcytoplasmic polyadenylationoogenesisegg activationDrosophila
Abstract
Eukaryotic mRNAs are subject to multiple types of tailing that critically influence mRNA stability and translatability. To investigate RNA tails at the genomic scale, we previously developed TAIL-seq, but its low sensitivity precluded its application to biological materials of minute quantity. In this study, we report a new version of TAIL-seq (mRNA TAIL-seq [mTAIL-seq]) with enhanced sequencing depth for mRNAs (by similar to 1000-fold compared with the previous version). The improved method allows us to investigate the regulation of poly(A) tails in Drosophila oocytes and embryos. We found that maternal mRNAs are polyadenylated mainly during late oogenesis, prior to fertilization, and that further modulation occurs upon egg activation. Wispy, a noncanonical poly(A) polymerase, adenylates the vast majority of maternal mRNAs, with a few intriguing exceptions such as ribosomal protein transcripts. By comparing mTAIL-seq data with ribosome profiling data, we found a strong coupling between poly(A) tail length and translational efficiency during egg activation. Our data suggest that regulation of poly(A) tails in oocytes shapes the translatomic landscape of embryos, thereby directing the onset of animal development. By virtue of the high sensitivity, low cost, technical robustness, and broad accessibility, mTAIL-seq will be a potent tool to improve our understanding of mRNA tailing in diverse biological systems.
ISSN
0890-9369
URI
https://hdl.handle.net/10371/171896
DOI
https://doi.org/10.1101/gad.284802.116
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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