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Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)

Cited 225 time in Web of Science Cited 229 time in Scopus
Authors
Ryoo, Soo-Ryoon; Lee, Jieon; Yeo, Jinah; Na, Hee-Kyung; Kim, Young-Kwan; Jang, Hongje; Lee, Jung Hyun; Han, Sang Woo; Lee, Younghoon; Kim, V. Narry; Min, Dal-Hee
Issue Date
2013-07
Citation
ACS Nano, Vol.7 No.7, pp.5882-5891
Keywords
biosensorgraphene oxidenanotechnologypeptide nucleic acidRNA recognition
Abstract
MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as important biomarkers since abnormal expression of specific miRNAs is associated with many diseases including cancer and diabetes. Therefore, it is important to develop biosensors to quantitatively detect miRNA expression levels. Here, we develop a nanosized graphene oxide (NGO) based miRNA sensor, which allows quantitative monitoring of target miRNA expression levels in living cells. The strategy is based on tight binding of NGO with peptide nucleic acid (PNA) probes, resulting in fluorescence quenching of the dye that is conjugated to the PNA, and subsequent recovery of the fluorescence upon addition of target miRNA. PNA as a probe for miRNA sensing offers many advantages including high sequence specificity, high loading capacity on the NGO surface compared to DNA and resistance against nuclease-mediated degradation. The present miRNA sensor allowed the detection of specific target miRNAs with the detection limit as low as similar to 1 pM and the simultaneous monitoring of three different miRNAs in a living cell.
ISSN
1936-0851
URI
https://hdl.handle.net/10371/171915
DOI
https://doi.org/10.1021/nn401183s
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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