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The Architecture of SARS-CoV-2 Transcriptome

Cited 1309 time in Web of Science Cited 1416 time in Scopus
Authors

Kim, Dongwan; Lee, Joo-Yeon; Yang, Jeong-Sun; Kim, Jun Won; Kim, V. Narry; Chang, Hyeshik

Issue Date
2020-05
Publisher
Cell Press
Citation
Cell, Vol.181 No.4, pp.914-921.e10
Abstract
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitran-scriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nano pore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
ISSN
0092-8674
URI
https://hdl.handle.net/10371/171943
DOI
https://doi.org/10.1016/j.cell.2020.04.011
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Research Area Molecular Biology & Genetics

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