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Chemical RNA digestion enables robust RNA-binding site mapping at single amino acid resolution
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Jong Woo | - |
dc.contributor.author | Kwon, S. Chul | - |
dc.contributor.author | Na, Yongwoo | - |
dc.contributor.author | Kim, V. Narry | - |
dc.contributor.author | Kim, Jong-Seo | - |
dc.date.accessioned | 2021-01-31T08:15:11Z | - |
dc.date.available | 2021-01-31T08:15:11Z | - |
dc.date.created | 2020-10-14 | - |
dc.date.created | 2020-10-14 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | Nature Structural and Molecular Biology, Vol.27 No.7, pp.678-682 | - |
dc.identifier.issn | 1545-9993 | - |
dc.identifier.other | 113025 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171946 | - |
dc.description.abstract | RNA-binding sites (RBSs) can be identified by liquid chromatography and tandem mass spectrometry analyses of the protein-RNA conjugates created by crosslinking, but RBS mapping remains highly challenging due to the complexity of the formed RNA adducts. Here, we introduce RBS-ID, a method that uses hydrofluoride to fully cleave RNA into mono-nucleosides, thereby minimizing the search space to drastically enhance coverage and to reach single amino acid resolution. Moreover, the simple mono-nucleoside adducts offer a confident and quantitative measure of direct RNA-protein interaction. Using RBS-ID, we profiled similar to 2,000 human RBSs and probedStreptococcus pyogenesCas9 to discover residues important for genome editing. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Chemical RNA digestion enables robust RNA-binding site mapping at single amino acid resolution | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김빛내리 | - |
dc.identifier.doi | 10.1038/s41594-020-0436-2 | - |
dc.citation.journaltitle | Nature Structural and Molecular Biology | - |
dc.identifier.wosid | 000539643200002 | - |
dc.identifier.scopusid | 2-s2.0-85086165211 | - |
dc.citation.endpage | 682 | - |
dc.citation.number | 7 | - |
dc.citation.startpage | 678 | - |
dc.citation.volume | 27 | - |
dc.identifier.sci | 000539643200002 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PROTEOME | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | DOMAINS | - |
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