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Bringing single-molecule spectroscopy to macromolecular protein complexes

Cited 19 time in Web of Science Cited 18 time in Scopus
Authors
Joo, Chirlmin; Fareh, Mohamed; Kim, V. Narry
Issue Date
2013-01
Citation
Trends in Biochemical Sciences, Vol.38 No.1, pp.30-37
Keywords
single-molecule fluorescencemacromolecular complexcell lysatesingle-molecule immunoprecipitationsingle-molecule pull-downprotein
Abstract
Single-molecule fluorescence spectroscopy offers real-time, nanometer-resolution information. Over the past two decades, this emerging single-molecule technique has been rapidly adopted to investigate the structural dynamics and biological functions of proteins. Despite this remarkable achievement, single-molecule fluorescence techniques must be extended to macromolecular protein complexes that are physiologically more relevant for functional studies. In this review, we present recent major breakthroughs for investigating protein complexes within cell extracts using single-molecule fluorescence. We outline the challenges, future prospects and potential applications of these new single-molecule fluorescence techniques in biological and clinical research.
ISSN
0968-0004
URI
https://hdl.handle.net/10371/171956
DOI
https://doi.org/10.1016/j.tibs.2012.10.005
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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