Publications

Detailed Information

Structure of human DROSHA

Cited 153 time in Web of Science Cited 159 time in Scopus
Authors

Kwon, S. Chul; Tuan Anh Nguyen; Choi, Yeon-Gil; Jo, Myung Hyun; Hohng, Sungchul; Kim, V. Narry; Woo, Jae-Sung

Issue Date
2016-01
Publisher
Cell Press
Citation
Cell, Vol.164 No.1-2, pp.81-90
Abstract
MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray structure of DROSHA in complex with the C-terminal helix of DGCR8. We find that DROSHA contains two DGCR8-binding sites, one on each RNase III domain (RIIID), which mediate the assembly of Microprocessor. The overall structure of DROSHA is surprisingly similar to that of Dicer despite no sequence homology apart from the C-terminal part, suggesting that DROSHA may have evolved from a Dicer homolog. DROSHA exhibits unique features, including non-canonical zinc-finger motifs, a long insertion in the first RIIID, and the kinked link between Connector helix and RIIID, which explains the 11-bp-measuring "ruler" activity of DROSHA. Our study implicates the evolutionary origin of DROSHA and elucidates the molecular basis of Microprocessor assembly and primary microRNA processing.
ISSN
0092-8674
URI
https://hdl.handle.net/10371/171963
DOI
https://doi.org/10.1016/j.cell.2015.12.019
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Natural Sciences
  • School of Biological Sciences
Research Area Molecular Biology & Genetics

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share