Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1
- Im, Young Jun; Park, Chung-Mo; Kim, Jeong-Il; Yang, Song-Sook; Kang, Jeong-Gu; Rho, Seong-Hwan; Kim, Jae Il; Song, Woo Keun; Song, Pill-Soon; Eom, Soo Hyun
- Issue Date
- Acta Crystallographica Section D: Biological Crystallography, Vol.56, pp.1446-1448
- The key response-regulator gene of light regulation, rcp1, from Synechocystis sp. has been overexpressed, purified and subsequently crystallized using ammonium sulfate as a precipitant in forms suitable for X-ray crystallographic studies. A native data set was collected to a resolution of 2.5 Angstrom at cryogenic temperature. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.04 (5), c = 60.29 (3) Angstrom. The Matthews parameter suggests that Rcp1 crystallizes with two molecules per asymmetric unit.
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