A Highly Selective and Sensitive Fluorescence Sensing System for Distinction between Diphosphate and Nucleoside Triphosphates

Abstract

Among the numerous chemosensors available for diphosphate (P2O74-, PPi) and nucleoside triphosphates (NTPs), only a few can distinguish between PPi and NTPs. Hence, very few bioanalytical applications based on such selective chemosensors have been realized. We have developed a new fluorescence sensing system for distinction between PPi and NTPs based on the combination of two sensors, a binuclear Zn(II) complex (1 center dot 2Zn) and boronic acid (BA), in which one chemosensor (1 center dot 2Zn) shows signal changes depending on the PPi (or NTP) concentration, and the other (BA) blocks the signal change caused by NTPs; this system enables the distinction of PPi from NTPs and is sensitive to nano-molar concentrations of PPi. The new sensing system has been successfully used for the direct quantification of RNA polymerase activity.