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A Highly Selective and Sensitive Fluorescence Sensing System for Distinction between Diphosphate and Nucleoside Triphosphates

Cited 45 time in Web of Science Cited 46 time in Scopus
Authors
Lee, Jae Han; Jeong, A. Reum; Jung, Jae-Hoon; Park, Chung-Mo; Hong, Jong-In
Issue Date
2011-01
Citation
Journal of Organic Chemistry, Vol.76 No.2, pp.417-423
Abstract
Among the numerous chemosensors available for diphosphate (P2O74-, PPi) and nucleoside triphosphates (NTPs), only a few can distinguish between PPi and NTPs. Hence, very few bioanalytical applications based on such selective chemosensors have been realized. We have developed a new fluorescence sensing system for distinction between PPi and NTPs based on the combination of two sensors, a binuclear Zn(II) complex (1 center dot 2Zn) and boronic acid (BA), in which one chemosensor (1 center dot 2Zn) shows signal changes depending on the PPi (or NTP) concentration, and the other (BA) blocks the signal change caused by NTPs; this system enables the distinction of PPi from NTPs and is sensitive to nano-molar concentrations of PPi. The new sensing system has been successfully used for the direct quantification of RNA polymerase activity.
ISSN
0022-3263
URI
https://hdl.handle.net/10371/171994
DOI
https://doi.org/10.1021/jo1017102
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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