Browse

Processing and secretion of a virally encoded antifungal toxin in transgenic tobacco plants: Evidence for a Kex2p pathway in plants

Cited 48 time in Web of Science Cited 47 time in Scopus
Authors
Kinal, Hai; Park, Chung-Mo; Berry, James O.; Koltin, Yigal; Bruenn, Jeremy A.
Issue Date
1995-06
Citation
Plant Cell, Vol.7 No.6, pp.677-688
Abstract
Ustilago maydis is a fungal pathogen of maize. Some strains of U. maydis encode secreted polypeptide toxins capable of killing other susceptible strains of U. maydis. We show here that one of these toxins, the KP6 killer toxin, is synthesized by transgenic tobacco plants containing the viral toxin cDNA under the control of a cauliflower mosaic virus promoter. The two components of the KP6 toxin, designated alpha and beta, with activity and specificity identical to those found in toxin secreted by U. maydis cells, were isolated from the intercellular fluid of the transgenic tobacco plants. The beta polypeptide from tobacco was identical in size and N-terminal sequence to the U.maydis KP6 beta polypeptide. Processing of the KP6 preprotoxin in U.maydis requires a subtilisin-like processing protease, Kex2p, which is present in both animal and fungal cells and is required for processing of (among other things) small secreted polypeptide hormones and secreted toxins. Our findings present evidence for Kex2p-like processing activity in plants. The systemic production of this viral killer toxin in crop plants may provide a new method of engineering biological control of fungal pathogens in crop plants.
ISSN
1040-4651
URI
https://hdl.handle.net/10371/172026
DOI
https://doi.org/10.1105/tpc.7.6.677
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse