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Alternative splicing provides a proactive mechanism for the diurnal CONSTANS dynamics in Arabidopsis photoperiodic flowering

Cited 12 time in Web of Science Cited 13 time in Scopus
Authors
Gil, Kyung-Eun; Park, Mi-Jeong; Lee, Hyo-Jun; Park, Young-Joon; Han, Shin-Hee; Kwon, Young-Ju; Seo, Pil Joon; Jung, Jae-Hoon; Park, Chung-Mo
Issue Date
2017-01
Citation
Plant Journal, Vol.89 No.1, pp.128-140
Keywords
alternative splicingphotoperiodic floweringCONSTANSE3 ubiquitin ligasesArabidopsis thaliana
Abstract
The circadian clock control of CONSTANS (CO) transcription and the light-mediated stabilization of its encoded protein coordinately adjust photoperiodic flowering by triggering rhythmic expression of the floral integrator flowering locus T (FT). Diurnal accumulation of CO is modulated sequentially by distinct E3 ubiquitin ligases, allowing peak CO to occur in the late afternoon under long days. Here we show that CO abundance is not simply targeted by E3 enzymes but is also actively self-adjusted through dynamic interactions between two CO isoforms. Alternative splicing of CO produces two protein variants, the full-size CO alpha and the truncated CO beta lacking DNA-binding affinity. Notably, CO beta, which is resistant to E3 enzymes, induces the interaction of CO alpha with CO-destabilizing E3 enzymes but inhibits the association of CO alpha with CO-stabilizing E3 ligase. These observations demonstrate that CO plays an active role in sustaining its diurnal accumulation dynamics during Arabidopsis photoperiodic flowering.
ISSN
0960-7412
URI
https://hdl.handle.net/10371/172040
DOI
https://doi.org/10.1111/tpj.13351
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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