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Targeted inactivation of transcription factors by overexpression of their truncated forms in plants

Cited 18 time in Web of Science Cited 20 time in Scopus
Authors
Seo, Pil Joon; Hong, Shin-Young; Ryu, Jae Yong; Jeong, Eun-Young; Kim, Sang-Gyu; Baldwin, Ian T.; Park, Chung-Mo
Issue Date
2012-10
Citation
Plant Journal, Vol.72 No.1, pp.162-172
Keywords
Arabidopsisartificial siPEPBrachypodiumpeptide interference (PEPi)transcription factortechnical advance
Abstract
Transcription factors are central constituents of gene regulatory networks that control diverse aspects of plant development and environmental adaptability. Therefore they have been explored for decades as primary targets for agricultural biotechnology. A gene of interest can readily be introduced into many crop plants, whereas targeted gene inactivation is practically difficult in many cases. Here, we developed an artificial small interfering peptide (a-siPEP) approach, which is based on overexpression of specific protein domains, and evaluated its application for the targeted inactivation of transcription factors in the dicot model, Arabidopsis, and monocot model, Brachypodium. We designed potential a-siPEPs of two representative MADS box transcription factors, SUPPRESSOR OF OVEREXPRESSOR OF CONSTANS 1 (SOC1) and AGAMOUS (AG), and a MYB transcription factor, LATE ELONGATED HYPOCOTYL (LHY). Transgenic plants overproducing the a-siPEPs displayed phenotypes comparable to those of gene-deficient mutants. The a-siPEPs attenuate nuclear import and DNA-binding of target transcription factors. Our data demonstrate that the a-siPEP tool is an efficient genetic means of inactivating specific transcription factors in plants.
ISSN
0960-7412
URI
https://hdl.handle.net/10371/172041
DOI
https://doi.org/10.1111/j.1365-313X.2012.05069.x
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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